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JA Clinical Reports

Table 2 Primer and probe sequences used in the nanopore-mediated genotyping of the six target SNPs

From: Rapid detection of single nucleotide polymorphisms using the MinION nanopore sequencer: a feasibility study for perioperative precision medicine

SNP

Locus-specific primer sequence (5′ to 3′) F, forward; R, reverse

PCR product (bp)

Probe sequence

rs1045642

F:

R:

ACTAACCCAAACAGGAAGTGTGG

GTGTGCTGGTCCTGAAGTTGA

413

CCTCACNATCTCT

rs1799971

F:

R:

AAGGTGGGAGGGGGCTATAC

ACTTCTCTGCTCCTGAAATTTTGAA

738

TAGATGGCNACCT

rs2165870

F:

R:

AGCTAATGCAGCTACTAGTTAA

TGCTATACATCACATCCTCAAGT

927

AGCCTGNTATACT

rs4369876

F:

R:

TTTGTCCACGCTGCTTCCAAAAC

TGCTGGTTTGTATTGTGGCCT

438

TTTCANATAATTT

rs33985936

F:

R:

TGGGTATCAAAGGGCAGCCA

AGCACTGGATCGATTCCGCC

466

CATGCCTGANGCC

rs140124801

F:

R:

CACCAGGTGGTCTATGCGGG

TACGTGGCCGAGAAGGAGTC

481

CGTAGCCCANCGT

  1. The locus-specific inner primer pairs used in the first PCR contained the following 5′ tail sequences: forward, TTTCTGTTGGTGCTGATATTGC + locus-specific sequence (F); reverse, ACTTGCCTGTCGCTCTATCTTC + locus-specific sequence (R). The base corresponding to the SNP site in each probe is represented by an “N” in each probe sequence
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